B-Raf Antibody (IHC600) - V600-E Mutation

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00517

Novus Biologicals, part of Bio-Techne
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NBP3-00517-1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # IHC600

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all B-Raf Antibodies »

Product Summary for B-Raf Antibody (IHC600) - V600-E Mutation

Immunogen

A peptide covering the V600E mutation.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for B-Raf Antibody (IHC600) - V600-E Mutation

Immunohistochemistry-Paraffin: B-Raf Antibody (IHC600) - V600-E Mutation [NBP3-00517]

Immunohistochemistry-Paraffin: B-Raf Antibody (IHC600) - V600-E Mutation [NBP3-00517]

Immunohistochemistry-Paraffin: B-Raf Antibody (IHC600) - V600-E Mutation [NBP3-00517] - Staining of B-Raf Antibody (IHC600) in thyroid papillary carcinoma with BRAF V600-E mutation

Applications for B-Raf Antibody (IHC600) - V600-E Mutation

Application
Recommended Usage

Immunohistochemistry

1:30

Immunohistochemistry-Paraffin

1:30
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Colorectal Adenocarcinoma, Thyroid Papillary Carcinoma with the BRAF V600E Mutation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Tris Buffer, pH 7.3 - 7.7, 1% BSA

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (IHC600) - V600-E Mutation

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B-Raf Antibody (IHC600) - V600-E Mutation

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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