B-Raf Antibody (RM8) - V600-E Mutation

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61503

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61503
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM8

Concentration

1 mg/ml

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Product Summary for B-Raf Antibody (RM8) - V600-E Mutation

Immunogen

A peptide corresponding to B-Raf V600E mutant

Specificity

This antibody reacts to the B-Raf V600E mutant. No cross reactivity with wild type BRAF.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B-Raf Antibody (RM8) - V600-E Mutation

Western Blot: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] -

Western Blot: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using NBP2-61503 at 2 ug/ml.

Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] -

Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections using NBP2-61503 at 2 ug/ml
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503]

Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503]

Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Negative for BRAF V600E.
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Applications for B-Raf Antibody (RM8) - V600-E Mutation

Application
Recommended Usage

ELISA

0.5-2 ug/ml

Immunocytochemistry/ Immunofluorescence

0.5-5 ug/ml

Immunohistochemistry

0.5-5 ug/mL

Western Blot

0.5-2ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS with 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (RM8) - V600-E Mutation

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B-Raf Antibody (RM8) - V600-E Mutation

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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