B220/CD45R Antibody (HIS24) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44007
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # HIS24
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD45R.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for B220/CD45R Antibody (HIS24) [FITC]
Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [FITC] [NBP1-44007] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).
Flow Cytometry: B220/CD45R Antibody (HIS24) [FITC] [NBP1-44007] - Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of Anti-Rat CD45R (B220) FITC (filled histogram). Total viable cells were used for analysis.
Applications for B220/CD45R Antibody (HIS24) [FITC]
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry-Frozen
10 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: B220/CD45R
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for B220/CD45R Antibody (HIS24) [FITC]
Product Specific Notices for B220/CD45R Antibody (HIS24) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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