B220/CD45R Antibody (PTPRC/1783R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53303

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-53303-100ug
NBP2-53303-20ug
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PTPRC/1783R

Concentration

0.2 mg/ml

View all B220/CD45R Antibodies »

Product Specifications

Immunogen

Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )

Localization

Cell Surface and Cytoplasmic

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54578)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for B220/CD45R Antibody (PTPRC/1783R)

Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303]

Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.
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Applications for B220/CD45R Antibody (PTPRC/1783R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-53303 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: B220/CD45R

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

UniProt

P08575

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (PTPRC/1783R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B220/CD45R Antibody (PTPRC/1783R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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