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B3GNT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88954

Novus Biologicals, part of Bio-Techne
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NBP1-88954
NBP1-88954-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for B3GNT5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B3GNT5 Antibody

Immunocytochemistry/ Immunofluorescence: B3GNT5 Antibody [NBP1-88954]

Immunocytochemistry/ Immunofluorescence: B3GNT5 Antibody [NBP1-88954]

Immunocytochemistry/Immunofluorescence: B3GNT5 Antibody [NBP1-88954] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Immunohistochemistry-Paraffin: B3GNT5 Antibody [NBP1-88954]

Immunohistochemistry-Paraffin: B3GNT5 Antibody [NBP1-88954]

Immunohistochemistry-Paraffin: B3GNT5 Antibody [NBP1-88954] - Staining of human liver shows moderate granular positivity in hepatocytes.

Applications for B3GNT5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B3GNT5

The B3GNT5 gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membraneprotein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likelycandidate for lactotri

Alternate Names

B3GN-T5, beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase, beta-1,3-Gn-T5, Beta-1,3-N-acetylglucosaminyltransferase 5, beta-1,3-N-acetylglucosaminyltransferase bGnT-5, Beta3Gn-T5, BGnT-5, EC 2.4.1, EC 2.4.1.-, Lactotriaosylceramide synthase, Lc(3)Cer synthase, Lc3 synthase, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5

Gene Symbol

B3GNT5

Additional B3GNT5 Products

Product Documents for B3GNT5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B3GNT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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