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B4GALNT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88662

Novus Biologicals, part of Bio-Techne
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NBP1-88662
NBP1-88662-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAPEPRYAHIPVRIKEQVVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLSRSQSPADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQAASGQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B4GALNT1 Antibody

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662]

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662]

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebellum, cerebral cortex, lymph node and pancreas using Anti-B4GALNT1 antibody NBP1-88662 (A) shows similar protein distribution across tissues to independent antibody NBP1-88663 (B).
Western Blot: B4GALNT1 Antibody [NBP1-88662]

Western Blot: B4GALNT1 Antibody [NBP1-88662]

Western Blot: B4GALNT1 Antibody [NBP1-88662] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662]

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662]

Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for B4GALNT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM2/GD2 Synthase/ B4GALNT1

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.

Long Name

Beta-1,4-N-Acetyl-Galactosaminyltransferase 1

Alternate Names

GALGT, GALNACT, GD2 Synthase, GM2 Synthase, SPG26

Gene Symbol

B4GALNT1

Additional GM2/GD2 Synthase/ B4GALNT1 Products

Product Documents for B4GALNT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B4GALNT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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