B7-1/CD80 Antibody (16-10A1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43385

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-43385-0.05mg
NBP1-43385-0.1mg
NBP1-43385-0.5mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 16-10A1

Format

BSA Free

Concentration

0.5 mg/ml

View all B7-1/CD80 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD80.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for B7-1/CD80 Antibody (16-10A1) - BSA Free

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
View 3 more images

Applications for B7-1/CD80 Antibody (16-10A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B7-1/CD80

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Alternate Names

B71, CD80

Gene Symbol

CD80

Additional B7-1/CD80 Products

Product Documents for B7-1/CD80 Antibody (16-10A1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for B7-1/CD80 Antibody (16-10A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.