B7-1/CD80 Antibody (16-10A1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43385
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0005.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]")
![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0007.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Feline
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # 16-10A1
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD80.
Reactivity Notes
Feline reactivity reported from a verified customer review.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for B7-1/CD80 Antibody (16-10A1) - BSA Free
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0001.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]")
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0004.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]")
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]
Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.![Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]](https://resources.bio-techne.com/images/products/B7-1-CD80-Antibody-16-10A1-Flow-Cytometry-NBP1-43385-img0006.jpg "Flow Cytometry: B7-1/CD80 Antibody (16-10A1) - BSA Free [NBP1-43385]")
Applications for B7-1/CD80 Antibody (16-10A1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: B7-1/CD80
Alternate Names
B71, CD80
Gene Symbol
CD80
Additional B7-1/CD80 Products
Product Documents for B7-1/CD80 Antibody (16-10A1) - BSA Free
Product Specific Notices for B7-1/CD80 Antibody (16-10A1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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