Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B7H6/4821

Format

Azide and BSA Free

Concentration

1 mg/ml

View all B7-H6 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa38-169) of human B7-H6 protein (exact sequence is proprietary) (Uniprot: Q68D85 )

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07759).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for B7-H6 Antibody (B7H6/4821) - Azide and BSA Free

Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339]

Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339]

Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339] - Formalin-fixed, paraffin-embedded human liver carcinoma in colon stained with B7-H6 Mouse Monoclonal Antibody (B7H6/4821).
Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339]

Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339]

Immunohistochemistry-Paraffin: B7-H6 Antibody (B7H6/4821) - Azide and BSA Free [NBP3-08339] - Formalin-fixed, paraffin-embedded human tonsil stained with B7-H6 Mouse Monoclonal Antibody (B7H6/4821).

Applications for B7-H6 Antibody (B7H6/4821) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: B7-H6

B7-H6 is a transmembrane immune regulatory protein that binds to NKp30 expressed on NK cells. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30-dependent cell lysis.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Gene Symbol

NCR3LG1

Additional B7-H6 Products

Product Documents for B7-H6 Antibody (B7H6/4821) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for B7-H6 Antibody (B7H6/4821) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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