BACE-1 Antibody (JE30-40)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32066
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE30-40
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human BACE-1 aa 450-501 / 501. (Uniprot: P56817)
Localization
Cell membrane, Golgi apparatus, trans-Golgi network, Endoplasmic reticulum, Endosome, Cell surface, Cytoplasmic vesicle membrane, Membrane raft, Lysosome, Late endosome, Early endosome, Recycling endosome, Cell projection, axon, dendrite.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BACE-1 Antibody (JE30-40)
Western Blot: BACE-1 Antibody (JE30-40) [NBP3-32066]
Western Blot: BACE-1 Antibody (JE30-40) [NBP3-32066] - Western blot analysis of BACE-1 on different lysates with Rabbit anti-BACE-1 antibody (NBP3-32066) at 1/1,000 dilution.Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Human cerebellum tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 56 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32066) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for BACE-1 Antibody (JE30-40)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BACE-1
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Alternate Names
BACE1
Gene Symbol
BACE1
Additional BACE-1 Products
Product Documents for BACE-1 Antibody (JE30-40)
Product Specific Notices for BACE-1 Antibody (JE30-40)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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