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BACH1 Antibody (OTI4E11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70240

Novus Biologicals, part of Bio-Techne
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NBP2-70240
Conjugate
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human BACH1(NP_996749) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BACH1 Antibody (OTI4E11) - Azide and BSA Free

Western Blot: BACH1 Antibody (OTI4E11)Azide and BSA Free [NBP2-70240]

Western Blot: BACH1 Antibody (OTI4E11)Azide and BSA Free [NBP2-70240]

Western Blot: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BACH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BACH1.
Immunohistochemistry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240]

Immunohistochemistry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240]

Immunohistochemistry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody.
Flow Cytometry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240]

Flow Cytometry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240]

Flow Cytometry: BACH1 Antibody (OTI4E11) - Azide and BSA Free [NBP2-70240] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BACH1 antibody, and then analyzed by flow cytometry.

Applications for BACH1 Antibody (OTI4E11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BACH1

BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.

Long Name

BTB and CNC homolog 1

Alternate Names

BTB, HA2303

Gene Symbol

BACH1

Additional BACH1 Products

Product Documents for BACH1 Antibody (OTI4E11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BACH1 Antibody (OTI4E11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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