Bad [p Ser112] Antibody (10T3G1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16914
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10T3G1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Bad [p Ser112] Antibody (10T3G1)
Immunogen
A synthetic phosphorylated peptide around S112 of human Bad (Q92934).
Modification
p Ser 112
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Bad [p Ser112] Antibody (10T3G1)
Western Blot: Bad [p Ser112] Antibody (10T3G1) [NBP3-16914] - Western blot analysis of extracts of NIH/3T3 cells, using Bad Rabbit mAb (NBP3-16914) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
Applications for Bad [p Ser112] Antibody (10T3G1)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bad
Long Name
Bcl-xL/Bcl-2 Associated Death Promoter
Alternate Names
BBC6, BCL2L8
Gene Symbol
BAD
Additional Bad Products
Product Documents for Bad [p Ser112] Antibody (10T3G1)
Product Specific Notices for Bad [p Ser112] Antibody (10T3G1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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