Bad Antibody (1I10A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15801

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15801-100ul
NBP3-15801-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1I10A2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bad Antibodies »

Product Summary for Bad Antibody (1I10A2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bad (Q92934). MFQIPEFEPSEQEDSSSAERGLGPSPAGDGPSGSGKHHRQAPGLLWDASHQQEQPTSSSHHGGAGAVEIRSRHSSYPAGTEDDEGMGEEPSPFRGRSRSA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bad Antibody (1I10A2)

Western Blot: Bad Antibody (1I10A2) [NBP3-15801]

Western Blot: Bad Antibody (1I10A2) [NBP3-15801]

Western Blot: Bad Antibody (1I10A2) [NBP3-15801] - Western blot analysis of extracts of various cell lines, using Bad antibody (NBP3-15801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Bad Antibody (1I10A2) [NBP3-15801]

Immunohistochemistry-Paraffin: Bad Antibody (1I10A2) [NBP3-15801]

Immunohistochemistry-Paraffin: Bad Antibody (1I10A2) [NBP3-15801] - Immunohistochemistry of paraffin-embedded human placenta using Bad antibody (NBP3-15801) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Bad Antibody (1I10A2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Bad Antibody (1I10A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bad Antibody (1I10A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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