Bad Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56079

Novus Biologicals, part of Bio-Techne
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NB100-56079
NB100-56079SS
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

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Product Specifications

Immunogen

A synthetic peptide corrsponding to amino acids 2-23 (GTPKQPSLAPAHALGLRKSDPG) of mouse/rat Bad was used as immunogen; GenBank nos. NP_031548.1/NP_073189.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bad Antibody

Immunohistochemistry-Paraffin: Bad Antibody [NB100-56079]

Immunohistochemistry-Paraffin: Bad Antibody [NB100-56079]

Immunohistochemistry-Paraffin: Bad Antibody [NB100-56079] - Analysis of a formalin fixed paraffin-embedded (FFPE) mouse epididymus tissue using 1:50 dilution of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIAR) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.

Applications for Bad Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bad

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both antiapoptotic (prosurvival) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of antiapoptotic and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies based on their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Antiapoptotic: Bcl-2, Bcl-xL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-xS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Antiapoptotic members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical antiapoptotic family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of antiapoptotic and proapoptotic proteins determines the susceptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Antiapoptotic proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. Mouse Bad is 204 amino acid protein and rat Bad is a 205 amino acid protein.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Entrez Gene IDs

12015 (Mouse); 64639 (Rat)

Gene Symbol

BAD

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Product Documents for Bad Antibody

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Certificate of Analysis

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Product Specific Notices for Bad Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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