BAF180/PB1 Antibody (PSH02-34)

Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067]

Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32067) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067]

Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180/PB1 with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32067, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067]

Western Blot: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Western blot analysis of BAF180/PB1 on different lysates with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 193 kDa
Observed band size: 193 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.