BAF180/PB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79832

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot (Negative)

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BAF180/PB1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human polybromo 1 (BRG1-associated factor 180) using the numbering given in entry AAP34197.1 (GeneID 55193).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF180/PB1 Antibody

Immunoprecipitation: BAF180/PB1 Antibody [NB100-79832]

Immunoprecipitation: BAF180/PB1 Antibody [NB100-79832]

Immunoprecipitation: BAF180/PB1 Antibody [NB100-79832] - Detection of Human PB1/BAF180 by Western Blot of Immunoprecipitate. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PB1/BAF180 antibody NB100-79832 used for IP at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time 10 seconds.
BAF180/PB1 Antibody [NB100-79832] - Detection of human BAF180/PB1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody NB100-79832 (lot NB100-79832-3) used for IP at 6 ug per reaction. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79832-2) and another rabbit anti-PBRM1 antibody. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BAF180/PB1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate
Application Notes
Western Blot Not recommended. Use rabbit anti-PBRM1 antibody NB100-79833.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BAF180/PB1

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PB1/BAF180 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PB1/BAF180 has been proposed to function as a tumor suppressor protein. Recently, PB1/BAF180 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21. Alternate names for PB1/BAF180 include protein polybromo-1, polybromo-1D, BRG1-associated factor 180, hPB1, and PBRM1.

Long Name

Protein polybromo-1

Alternate Names

BAF180, hPB1, PB1, PBRM1, Polybromo-1D

Entrez Gene IDs

55193 (Human)

Gene Symbol

PBRM1

UniProt

Q86U86 (Human)

Additional BAF180/PB1 Products

Product Documents for BAF180/PB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAF180/PB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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