BAF53A Antibody (3C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00000086-M10

Novus Biologicals, part of Bio-Techne
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H00000086-M10
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BAF53A Antibodies »

Product Specifications

Immunogen

ACTL6A (NP_817126, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHPVLM

Specificity

ACTL6A - actin-like 6A (3C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BAF53A Antibody (3C4)

Western Blot: BAF53A Antibody (3C4) [H00000086-M10]

Western Blot: BAF53A Antibody (3C4) [H00000086-M10]

Western Blot: BAF53A Antibody (3C4) [H00000086-M10] - Analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4. Lane 1: ACTL6A transfected lysatE (47.5 KDa). Lane 2: Non-transfected lysate.
ELISA: BAF53A Antibody (3C4) [H00000086-M10]

ELISA: BAF53A Antibody (3C4) [H00000086-M10]

ELISA: BAF53A Antibody (3C4) [H00000086-M10] - Detection limit for recombinant GST tagged ACTL6A is approximately 0.3ng/ml as a capture antibody.

Applications for BAF53A Antibody (3C4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BAF53A

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Entrez Gene IDs

86 (Human)

Gene Symbol

ACTL6A

UniProt

NP_817126 (Human)

Additional BAF53A Products

Product Documents for BAF53A Antibody (3C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAF53A Antibody (3C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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