BAF53A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1421

Novus Biologicals, part of Bio-Techne
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NB100-1421
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BAF53A Antibodies »

Product Specifications

Immunogen

Peptide with sequence YEEGGKQCVERKCP corresponding to C-Terminus according to NP_004292.1, NP_817126.1, NP_057272.1.

Epitope

From C terminus.

Localization

Nuclear

Specificity

This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Reported variants represent identical protein (NP_817126.1; NP_829888.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BAF53A Antibody

Western Blot: BAF53A Antibody [NB100-1421]

Western Blot: BAF53A Antibody [NB100-1421]

Western Blot: BAF53A Antibody [NB100-1421] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: BAF53A Antibody [NB100-1421]

Immunocytochemistry/ Immunofluorescence: BAF53A Antibody [NB100-1421]

Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-1421] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: BAF53A Antibody [NB100-1421]

Flow Cytometry: BAF53A Antibody [NB100-1421]

Flow Cytometry: BAF53A Antibody [NB100-1421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for BAF53A Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:6000

Western Blot

0.5 - 2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAF53A

BAF53A is a 53kd subunit of the BAF (BRG1/brm-associated factor) complex, a SWI/SNF chromatin remodeling and histone acetyltransferase complex that functions in the regulation of gene transcription. In the BAF complex, BAF53A is important to the ATPase activity of SMARCA4/BRG1 and the association of the BAF complex with chromatin. BAF53A also appears to be important to the formation of higher-order chromatin structure and has been shown to associate with mitotic chromosomes. Recently, BAF53A has been found to interact with p53 and repress p53-mediated gene transcription in a BRG1-independent manner.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Entrez Gene IDs

51412 (Human); 86 (Human)

Gene Symbol

ACTL6A

UniProt

NP_057272 (Human), O96019 (Human)

Additional BAF53A Products

Product Documents for BAF53A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAF53A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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