BAF53A Antibody

Western Blot: BAF53A Antibody [NB100-61628]

Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628]

Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: BAF53A Antibody [NB100-61628] -

Western Blot: BAF53A Antibody [NB100-61628] - BRD9 defines a distinct, non-canonical BAF complex. a Immunoprecipitation (IP)-mass spectrometry using BRD9 or IgG antibody from mouse ESCs. The plot shows the spectral count fold change in BRD9 IP relative to IgG & the corresponding AC test p values, calculated with two technical replicates using PatternLab. In orange are proteins that satisfy FC 2, AC test p < 0.05. b Immunoblotting analysis of fractions after mouse ESC nuclear lysates were subjected to a density sedimentation assay in 10–30% glycerol gradient. LMW & HMW indicate lower & higher molecular weights, respectively. Nonspecific bands are marked with an asterisk. Molecular weights from ladder are indicated. c Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, & BAF47. Blots developed using chemiluminescence are marked with double asterisks; each IP was taken from a different exposure. d Depletion IP experiment from ESCs using antibodies against BRG1 or BAF155. Each lane shows the remaining proteins after each successive IP, labeled 1 through 4. e IP experiment from ESCs using antibodies against IgG or BRD9, which was incubated in increasing concentration of urea. f Schematic of the esBAF, GBAF, & PBAF complexes in mouse ESCs. Colored in orange, pink, & mustard yellow are subunits that define each individual complex. In green is the enzymatic component, BRG1. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30510198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.