BAF53A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55378

Novus Biologicals, part of Bio-Techne
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NBP1-55378
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all BAF53A Antibodies »

Product Summary for BAF53A Antibody

Immunogen

Synthetic peptides corresponding to ACTL6A (actin-like 6A) The peptide sequence was selected from the N terminal of ACTL6A)(50ug). Peptide sequence VDFPTAIGMVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEA. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for BAF53A Antibody

Western Blot: BAF53A Antibody [NBP1-55378]

Western Blot: BAF53A Antibody [NBP1-55378]

Western Blot: BAF53A Antibody [NBP1-55378] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.
Western Blot: BAF53A Antibody [NBP1-55378]

Western Blot: BAF53A Antibody [NBP1-55378]

Western Blot: BAF53A Antibody [NBP1-55378] - Mouse WT brain and Rat brain, concentration 2ug/ml.

Applications for BAF53A Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAF53A

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Gene Symbol

ACTL6A

UniProt

O96019 (Human)

Additional BAF53A Products

Product Documents for BAF53A Antibody

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Certificate of Analysis

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Product Specific Notices for BAF53A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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