BAF53A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55376

Novus Biologicals, part of Bio-Techne
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NBP2-55376
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BAF53A Antibodies »

Product Summary for BAF53A Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: TVRAGYAGEDCPKVDFPTAIGMVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF53A Antibody

Western Blot: BAF53A Antibody [NBP2-55376]

Western Blot: BAF53A Antibody [NBP2-55376]

Western Blot: BAF53A Antibody [NBP2-55376] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.
Immunocytochemistry/ Immunofluorescence: BAF53A Antibody [NBP2-55376]

Immunocytochemistry/ Immunofluorescence: BAF53A Antibody [NBP2-55376]

Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NBP2-55376] - Staining of human cell line A-431 shows localization to nucleoplasm.

Applications for BAF53A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAF53A

BAF53A encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Alternate Names

actin-like 6A, actin-like protein 6A, actin-related protein 4,53 kDa BRG1-associated factor A, Actin-related protein Baf53a, Actl6, Arp4, ArpNbeta, BAF complex 53 kDa subunit, Baf53a, BAF53AACTL6, BAF53MGC5382, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80KARPN-BETA

Gene Symbol

ACTL6A

Additional BAF53A Products

Product Documents for BAF53A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAF53A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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