Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all BAF57 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 360 and the C-terminus (residue 411) of human BAF57/SMARCE1using the numbering given in entry NP_003070.3 (GeneID 6605).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAF57 Antibody - BSA Free

Western Blot: BAF57 Antibody [NB100-2591]

Western Blot: BAF57 Antibody [NB100-2591]

Western Blot: BAF57 Antibody [NB100-2591] - Detection of Human and Mouse BAF57/SMARCE1 on HeLa whole cell lysates using NB100-2591.
Immunohistochemistry: BAF57 Antibody [NB100-2591]

Immunohistochemistry: BAF57 Antibody [NB100-2591]

Immunohistochemistry: BAF57 Antibody [NB100-2591] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- BAF57/SMARCE1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for BAF57 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful for Western Blot and immunoprecipitation. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BAF57

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Alternate Names

BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Entrez Gene IDs

6605 (Human); 57376 (Mouse)

Gene Symbol

SMARCE1

UniProt

Q969G3 (Human)

Additional BAF57 Products

Product Documents for BAF57 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BAF57 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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