BAFF/BLyS/TNFSF13B Antibody (182)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89246

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 182

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BAFF/BLyS/TNFSF13B Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human BAFF/BLyS/TNFSF13B (Uniprot#: Q9Y275-1; Ala134-Leu285).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for BAFF/BLyS/TNFSF13B Antibody (182)

Western Blot: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Western Blot: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Western Blot: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Lane A: HL-60 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: MOLT-4 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDa Observed band size:32 kDa (We are unsure as to the identity of these extra bands.)
Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Analysis of Human BAFF(CD257) expression on U937 cells. Cells were stained with purified anti-Human BAFF(CD257), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Immunoprecipitation: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246]

Immunoprecipitation: BAFF/BLyS/TNFSF13B Antibody (182) [NBP2-89246] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-BAFF rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-BAFF rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 31 kDa

Applications for BAFF/BLyS/TNFSF13B Antibody (182)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAFF/BLyS/TNFSF13B

TNFSF13B recognizes membrane bound and soluble human and mouse(weak) BAFF.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Gene Symbol

TNFSF13B

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for BAFF/BLyS/TNFSF13B Antibody (182)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAFF/BLyS/TNFSF13B Antibody (182)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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