BAFF/BLyS/TNFSF13B Antibody (Buffy-2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97622
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgM Clone # Buffy-2
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human soluble BAFF (aa 83-285).
Reactivity Notes
There is a weak corss-reactivity to Mouse.
Specificity
Recognizes membrane-bound and soluble human and mouse (weak) BAFF.
Clonality
Monoclonal
Host
Rat
Isotype
IgM
Scientific Data Images for BAFF/BLyS/TNFSF13B Antibody (Buffy-2)
![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]](https://resources.bio-techne.com/images/products/BAFF-BLyS-TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0002.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]")
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Human skin with marked lymphocyte infiltration. Lymphocytes and lymphatic vessels were membranous or cytoplasmic positive for BAFF. Nuclei of keratinocytes were also stained. Buffy2 (working dilution 2ug/mL). detected with Leica BOND Refine polymer (DAB) kit. Rabbit ant-rat IgG+M as a linker antibody pretreatment: basic solution (BOND ER2). IHC-P image submitted by a verified customer review.![Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]](https://resources.bio-techne.com/images/products/TNFSF13B-Antibody-Buffy-2-Immunohistochemistry-Paraffin-NBP1-97622-img0001.jpg "Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]")
Immunohistochemistry-Paraffin: BAFF/BLyS/TNFSF13B Antibody (Buffy-2) [NBP1-97622]
Immunohistochemistry-Paraffin: TNFSF13B Antibody (Buffy-2) [NBP1-97622] - Staining of paraffin section of synovial tissue from a patient with Rheumatoid Arthritis. Arrows indicate vessels (20x) staining positive for BAFF using Buffy-2. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution.Applications for BAFF/BLyS/TNFSF13B Antibody (Buffy-2)
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Reviewed Applications
Read 1 review rated 5 using NBP1-97622 in the following applications:
Formulation, Preparation, and Storage
Purification
Thiophilic Chromatography
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BAFF/BLyS/TNFSF13B
Long Name
B cell Activating Factor
Alternate Names
BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4
Gene Symbol
TNFSF13B
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for BAFF/BLyS/TNFSF13B Antibody (Buffy-2)
Product Specific Notices for BAFF/BLyS/TNFSF13B Antibody (Buffy-2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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