BAG2 Antibody

Immunocytochemistry/ Immunofluorescence: BAG2 Antibody [NB100-56087]

Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NB100-56087] - Immunofluorescence confocal microscopy of BAG-2 using NB100-56087 at 1:2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.

Immunohistochemistry-Paraffin: BAG2 Antibody [NB100-56087]

Immunohistochemistry-Paraffin: BAG2 Antibody [NB100-56087] - BAG-2 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human BAG-2 polyclonal Antibody (Catalog # NB100-56087) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.