BAG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59087

Novus Biologicals, part of Bio-Techne
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NBP1-59087
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all BAG2 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to BAG2(BCL2-associated athanogene 2) The peptide sequence was selected from the C terminal of BAG2. Peptide sequence VDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSK. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26729625)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for BAG2 Antibody

Western Blot: BAG2 Antibody [NBP1-59087]

Western Blot: BAG2 Antibody [NBP1-59087]

Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml
Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087]

Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087]

Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: BAG2 Antibody [NBP1-59087]

Western Blot: BAG2 Antibody [NBP1-59087]

Western Blot: BAG2 Antibody [NBP1-59087] - Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human.
View 1 more images

Applications for BAG2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 31570704).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAG2

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Alternate Names

BAG family molecular chaperone regulator 2, BAG-2, BAG-family molecular chaperone regulator-2, Bcl-2-associated athanogene 2, BCL2-associated athanogene 2, dJ417I1.2, dJ417I1.2 (BAG-family molecular chaperone regulator 2), KIAA0576, MGC149462

Gene Symbol

BAG2

UniProt

O95816 (Human)

Additional BAG2 Products

Product Documents for BAG2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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