BAG3 Antibody (3D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26483
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human BAG3 [UniProt O95817]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BAG3 Antibody (3D8)
Immunohistochemistry: BAG3 Antibody (3D8) [NBP3-26483] -
Immunohistochemistry: BAG3 Antibody (3D8) [NBP3-26483] - Image of BAG3 Antibody (3D8) diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: BAG3 Antibody (3D8) [NBP3-26483] -
Western Blot: BAG3 Antibody (3D8) [NBP3-26483] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate.All lanes: BAG3 Antibody at 0.73ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 62 KDa
Observed band size: 80 KDa
Applications for BAG3 Antibody (3D8)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: BAG3
Alternate Names
BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307
Gene Symbol
BAG3
Additional BAG3 Products
Product Documents for BAG3 Antibody (3D8)
Product Specific Notices for BAG3 Antibody (3D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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