BAG5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56091

Novus Biologicals, part of Bio-Techne
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NB100-56091
NB100-56091SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine

Cited:

Human

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all BAG5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 168-187 (KKQPSLPLSEDAHPSVAKIN) of human BAG-5 was used as immunogen, GenBank no. gi|6631077|ref|NP_004864.1|. The amino acid sequence used for immunogen is 100% consevered with chicken and dog BAG-5, 95% conserved with mouse BAG-5, and 94% conserved with rat BAG-5.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAG5 Antibody

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091]

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091]

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091] - Formalin-fixed, paraffin-embedded human cervical tissue stained for BAG-5 expression using this antibody at 1:2000. A. normal cervix epithelium. B. cervical cancer. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091]

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091]

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091] - Formalin-fixed, paraffin-embedded human tissues stained for BAG-5 expression using this antibody at 1:2000. A. normal placenta. B. breast cancer. C. normal spleen. normal cervix epithelium. Hematoxylin-eosin counterstain.

Applications for BAG5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAG5

The BAG (Bcl-2-associated anthanogene) proteins are a family of chaperone regulators that modulate several diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70 and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG-family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (RAP46/HAP46), and BAG-1L, BAG-2, BAG-3 (CAIR-1/Bis,), BAG-4 (SODD), BAG-5 and BAG-6 (Scythe, BAT3). The following amino acids lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 amino acid, 34 kDa), BAG-1S (219 amino acid, 29 kDa), BAG-1M (274 amino acid, 46 kDa), BAG-1L (345 amino acid, 52 kDa), BAG-2 [212 amino acid; 24 kDa (Arndt et al. 2005)], BAG-3 (575 amino acid, 74 kDa), BAG-4 (456 amino acid; 60 kDa), BAG-5 ([442 amino acid; 51 kDa (Kalia et al. 2004)], and BAG-6 (1129 amino acid; 150 kDa).

Alternate Names

BAG family molecular chaperone regulator 5, BAG-5, BAG-family molecular chaperone regulator-5, Bcl-2-associated athanogene 5, BCL2-associated athanogene 5, KIAA0873

Entrez Gene IDs

9529 (Human); 70369 (Mouse); 366734 (Rat)

Gene Symbol

BAG5

Additional BAG5 Products

Product Documents for BAG5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAG5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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