BAK Antibody (38E2) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74026PE
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 38E2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human BAK (29-187aa) purified from E.coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for BAK Antibody (38E2) [PE]
Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Applications for BAK Antibody (38E2) [PE]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: BAK
Long Name
Bcl-2 Antagonist/Killer
Alternate Names
BAK1, BCL2L7
Gene Symbol
BAK1
Additional BAK Products
Product Documents for BAK Antibody (38E2) [PE]
Product Specific Notices for BAK Antibody (38E2) [PE]
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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