BAK Antibody (3K5Y9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15336

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15336
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3K5Y9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BAK Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 11-82 of human BAK (NP_001179.1). RQECGEPALPSASEEQVAQDTEEVFRSYVFYRHQQEQEAEGVAAPADPEMVTLPLQPSSTMGQVGRQLAIIG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BAK Antibody (3K5Y9)

Western Blot: BAK Antibody (3K5Y9) [NBP3-15336]

Western Blot: BAK Antibody (3K5Y9) [NBP3-15336]

Western Blot: BAK Antibody (3K5Y9) [NBP3-15336] - Western blot analysis of extracts of various cell lines, using (NBP3-15336) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for BAK Antibody (3K5Y9)

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAK

Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Gene Symbol

BAK1

Additional BAK Products

Product Documents for BAK Antibody (3K5Y9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAK Antibody (3K5Y9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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