Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BARD1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human BRCA1-associated RING Domain 1 using the numbering given in entry NP_000456.1 (GeneID 580).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BARD1 Antibody

Western Blot: BARD1 Antibody [NB100-319]

Western Blot: BARD1 Antibody [NB100-319]

Western Blot: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, Hep-G2, K-562, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BARD1 antibody NB100-319 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: BARD1 Antibody [NB100-319]

Immunoprecipitation: BARD1 Antibody [NB100-319]

Immunoprecipitation: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BARD1 antibody NB100-319 (NB100-319 lot 4) used for IP at 6 ug per reaction. BARD1 was also immunoprecipitated by a previous lot of this antibody (NB100-319 lot 3). For blotting immunoprecipitated BARD1, NB100-319 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for BARD1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500
Application Notes
*The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BARD1

BRCA1-Associated RING Domain gene 1 (BARD1) functions as a tumor suppressor, especially in breast and ovarian cancers. It plays a major role in cancer suppression when it forms a heterodimer with BRCA1. It also plays a role in mediating apoptotic stress and p53-dependent apoptosis.

Long Name

BRCA1 associated RING domain 1

Alternate Names

BARD-1, BRCA1 associated RING domain 1, BRCA1-associated RING domain gene 1, BRCA1-associated RING domain protein 1, EC 6.3.2.-

Entrez Gene IDs

580 (Human)

Gene Symbol

BARD1

UniProt

NP_000456.1 (Human)

Additional BARD1 Products

Product Documents for BARD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BARD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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