Bassoon Antibody (HL1732) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25376

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25376
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1732

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Bassoon Antibodies »

Product Specifications

Immunogen

Synthetic peptide of mouse Bassoon

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bassoon Antibody (HL1732) - Azide and BSA Free

Bassoon Antibody (HL1732) - Azide and BSA Free

Immunohistochemistry-Paraffin: Bassoon Antibody (HL1732) - Azide and BSA Free [NBP3-25376] -

Immunohistochemistry-Paraffin: Bassoon Antibody (HL1732) - Azide and BSA Free [NBP3-25376] - Bassoon antibody [HL1732] detects Bassoon protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Bassoon stained by Bassoon antibody [HL1732] (NBP3-25376) diluted at 1:100. Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min
Bassoon Antibody (HL1732) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Bassoon Antibody (HL1732) - Azide and BSA Free [NBP3-25376] -

Immunocytochemistry/Immunofluorescence: Bassoon Antibody (HL1732) - Azide and BSA Free [NBP3-25376] - Bassoon antibody [HL1732] detects Bassoon protein at vesicle by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bassoon stained by Bassoon antibody [HL1732] (NBP3-25376) diluted at 1:250. Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for Bassoon Antibody (HL1732) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Gene Symbol

BSN

Additional Bassoon Products

Product Documents for Bassoon Antibody (HL1732) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bassoon Antibody (HL1732) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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