BBX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47542

Novus Biologicals, part of Bio-Techne
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NBP2-47542
NBP2-47542-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BBX Antibodies »

Product Summary for BBX Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NSLPQYSPVTFDRKCVPVPRKKKKTGNVSSEPTKTSKGSGDKWSNKQLFLDAIHPTEAIFSEDRNTMEPVHKVKNIPSIFNTPEPTTTQEPLVGSQKRKARKTKI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BBX Antibody

Immunocytochemistry/ Immunofluorescence: BBX Antibody [NBP2-47542]

Immunocytochemistry/ Immunofluorescence: BBX Antibody [NBP2-47542]

Immunocytochemistry/Immunofluorescence: BBX Antibody [NBP2-47542] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542]

Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542]

Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542]

Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542]

Immunohistochemistry-Paraffin: BBX Antibody [NBP2-47542] - Analysis in human testis and skeletal muscle tissues. Corresponding BBX RNA-seq data are presented for the same tissues.
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Applications for BBX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BBX

Transcription factor that is necessary for cell cycle progression from G1 to S phase

Alternate Names

Bobby sox homolog, bobby sox homolog (Drosophila), HBP2HSPC339, HMG box transcription factor BBX, HMG box-containing protein 2, MDS001, x 001 protein

Gene Symbol

BBX

Additional BBX Products

Product Documents for BBX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BBX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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