BCAP31 Antibody (12G12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26481
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12G12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human BCAP31 [UniProt P51572]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BCAP31 Antibody (12G12)
Immunohistochemistry: BCAP31 Antibody (12G12) [NBP3-26481] -
Immunohistochemistry: BCAP31 Antibody (12G12) [NBP3-26481] - Image of BCAP31 Antibody (12G12) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: BCAP31 Antibody (12G12) [NBP3-26481] -
Western Blot: BCAP31 Antibody (12G12) [NBP3-26481] - Positive Western Blot detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate.All lanes: BCAP31 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 28, 35 kDa
Observed band size: 28-36 kDa
Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (12G12) [NBP3-26481] -
Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (12G12) [NBP3-26481] - Staining of A549 cell with BCAP31 Antibody (12G12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 526-conjugated Goat Anti-Rabbit IgG (H+L).Applications for BCAP31 Antibody (12G12)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: BAP31
Long Name
B-cell Receptor-associated Protein 31
Alternate Names
6C6-Ag, BCAP31
Gene Symbol
BCAP31
Additional BAP31 Products
Product Documents for BCAP31 Antibody (12G12)
Product Specific Notices for BCAP31 Antibody (12G12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...