BCAP31 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89357

Novus Biologicals, part of Bio-Techne
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NBP1-89357
NBP1-89357-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BAP31 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAP31 Antibody

Immunohistochemistry-Paraffin: BCAP31 Antibody [NBP1-89357]

Immunohistochemistry-Paraffin: BCAP31 Antibody [NBP1-89357]

Immunohistochemistry-Paraffin: BCAP31 Antibody [NBP1-89357] - Staining in human epididymis and skeletal muscle tissues using anti-BCAP31 antibody. Corresponding BCAP31 RNA-seq data are presented for the same tissues.
Western Blot: BCAP31 Antibody [NBP1-89357]

Western Blot: BCAP31 Antibody [NBP1-89357]

Western Blot: BCAP31 Antibody [NBP1-89357] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: BCAP31 Antibody [NBP1-89357]

Immunocytochemistry/ Immunofluorescence: BCAP31 Antibody [NBP1-89357]

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody [NBP1-89357] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
View 3 more images

Applications for BCAP31 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAP31

BAP31 (B Cell Receptor Associated Protein, 28 kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo and hetero oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL2/BCLXL associated protein. In the C terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.

Long Name

B-cell Receptor-associated Protein 31

Alternate Names

6C6-Ag, BCAP31

Gene Symbol

BCAP31

Additional BAP31 Products

Product Documents for BCAP31 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BCAP31 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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