BCAR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82523

Novus Biologicals, part of Bio-Techne
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NBP1-82523
NBP1-82523-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BCAR3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDDFSHMGTLPHSKSPRQNSPVTQDGIQESPWQDRHGETFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKKEL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAR3 Antibody

Western Blot: BCAR3 Antibody [NBP1-82523]

Western Blot: BCAR3 Antibody [NBP1-82523]

Western Blot: BCAR3 Antibody [NBP1-82523] - Analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: BCAR3 Antibody [NBP1-82523]

Immunocytochemistry/ Immunofluorescence: BCAR3 Antibody [NBP1-82523]

Immunocytochemistry/Immunofluorescence: BCAR3 Antibody [NBP1-82523] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and centrosome.
Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523]

Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523]

Immunohistochemistry-Paraffin: BCAR3 Antibody [NBP1-82523] - Staining of human kidney shows moderate cytoplasmic positivity in tubular cells.

Applications for BCAR3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCAR3

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq]

Alternate Names

breast cancer estrogen resistance 3, dJ1033H22.2 (breast cancer estrogen resistance 3), KIAA0554, Novel SH2-containing protein 2, NSP2SH2D3Bbreast cancer estrogen resistance protein 3, SH2 domain-containing protein 3B

Gene Symbol

BCAR3

Additional BCAR3 Products

Product Documents for BCAR3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BCAR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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