BCAS1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-681
![Western Blot: BCAS1 AntibodyBSA Free [NB100-681]](https://resources.bio-techne.com/images/products/BCAS1-Antibody-Western-Blot-NB100-681-img0005.jpg "Western Blot: BCAS1 AntibodyBSA Free [NB100-681]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Reacts with residues 532-550 [DTNSLQNGDKLQKRPEKRQ] of human NABC1.
Specificity
Reacts with human NABC1. Reactivity to other species unknown.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BCAS1 Antibody - BSA Free
Western Blot: BCAS1 AntibodyBSA Free [NB100-681]
Western Blot: BCAS1 Antibody [NB100-681] - Western blot analysis of BCAS1 from 15 ug of MCF-7 cell lysate using NB100-681 at 1:1000 dilution. BCAS1 is a 60 kDa protein: however it forms homodimers, which are detected in MCF-7 cells.![Western Blot: BCAS1 AntibodyBSA Free [NB100-681]](https://resources.bio-techne.com/images/products/BCAS1-Antibody-Western-Blot-NB100-681-img0004.jpg "Western Blot: BCAS1 AntibodyBSA Free [NB100-681]")
Western Blot: BCAS1 AntibodyBSA Free [NB100-681]
Western Blot: BCAS1 Antibody [NB100-681] - Detection of NABC1 in MCF-7 whole cell lysate (15 ug) using a 1:1,000 dilution of NB 100-681.![Simple Western: BCAS1 AntibodyBSA Free [NB100-681]](https://resources.bio-techne.com/images/products/BCAS1-Antibody-Simple-Western-NB100-681-img0003.jpg "Simple Western: BCAS1 AntibodyBSA Free [NB100-681]")
Simple Western: BCAS1 AntibodyBSA Free [NB100-681]
Simple Western: BCAS1 Antibody [NB100-681] - Simple Western lane view shows a specific band for BCAS1 in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for BCAS1 Antibody - BSA Free
Application
Recommended Usage
Simple Western
1:5000
Western Blot
1:100-1:2000
Application Notes
This antibody may be used for Western blot analysis where 2 bands are seen at ~120 and ~80 kDa. NABC1 is a 60 kDa protein; however it forms dimers, which are readily detectable in MCF-7 cells. No other applications have been tested. Suggested Working Dilutions:* Western blot - 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BCAS1
Alternate Names
breast carcinoma amplified sequence 1, breast carcinoma-amplified sequence 1, NABC1AIBC1Amplified and overexpressed in breast cancer, Novel amplified in breast cancer 1
Entrez Gene IDs
8537 (Human)
Gene Symbol
BCAS1
Additional BCAS1 Products
Product Documents for BCAS1 Antibody - BSA Free
Product Specific Notices for BCAS1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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