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BCAS2 Antibody (1D10)

Novus Biologicals, part of Bio-Techne | Catalog # H00010286-M01

Novus Biologicals, part of Bio-Techne
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H00010286-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

BCAS2 (NP_005863, 116 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Reactivity Notes

Human. Other species not tested.

Specificity

BCAS2 - breast carcinoma amplified sequence 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for BCAS2 Antibody (1D10)

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - BCAS2 monoclonal antibody (M01), clone 1D10 Analysis of BCAS2 expression in K-562.
Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 over-expressed 293 cell line, cotransfected with BCAS2 Validated Chimera RNAi ( Cat # H00010286-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BCAS2 monoclonal antibody (M01), clone 1D10 (Cat # H00010286-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01]

Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 expression in transfected 293T cell line by BCAS2 monoclonal antibody (M01), clone 1D10.Lane 1: BCAS2 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.

Applications for BCAS2 Antibody (1D10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BCAS2

BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.

Alternate Names

breast carcinoma amplified sequence 2, Breast carcinoma-amplified sequence 2, DAM1DNA amplified in mammary carcinoma 1 protein, pre-mRNA-splicing factor SPF27, Snt309, SPF27, spliceosome associated protein, amplified in breast cancer, Spliceosome-associated protein SPF 27

Entrez Gene IDs

10286 (Human)

Gene Symbol

BCAS2

OMIM

605783 (Human)

Additional BCAS2 Products

Product Documents for BCAS2 Antibody (1D10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BCAS2 Antibody (1D10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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