Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BCAS2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and the C-terminus (residue 225) of human Breast Cancer Amplified Sequence 2 using the numbering given in entry NP_005863.1 (GeneID 10286).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAS2 Antibody

Western Blot: BCAS2 Antibody [NB110-40681]

Western Blot: BCAS2 Antibody [NB110-40681]

Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
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Applications for BCAS2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB110-40681 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCAS2

BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.

Alternate Names

breast carcinoma amplified sequence 2, Breast carcinoma-amplified sequence 2, DAM1DNA amplified in mammary carcinoma 1 protein, pre-mRNA-splicing factor SPF27, Snt309, SPF27, spliceosome associated protein, amplified in breast cancer, Spliceosome-associated protein SPF 27

Entrez Gene IDs

10286 (Human); 68183 (Mouse)

Gene Symbol

BCAS2

UniProt

O75934 (Human)

Additional BCAS2 Products

Product Documents for BCAS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCAS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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