BCAT1 Antibody (OTI3F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70242

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BCAT1 Antibodies »

Product Summary for BCAT1 Antibody (OTI3F5) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCAT1 Antibody (OTI3F5) - Azide and BSA Free

Western Blot: BCAT1 Antibody (OTI3F5)Azide and BSA Free [NBP2-70242]

Western Blot: BCAT1 Antibody (OTI3F5)Azide and BSA Free [NBP2-70242]

Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.
Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242]

Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242]

Immunohistochemistry: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.
Western Blot: BCAT1 Antibody (OTI3F5)Azide and BSA Free [NBP2-70242]

Western Blot: BCAT1 Antibody (OTI3F5)Azide and BSA Free [NBP2-70242]

Western Blot: BCAT1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-70242] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)
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Applications for BCAT1 Antibody (OTI3F5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCAT1

BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic) is involved in catalyzing branched chain amino acids. BCAT1 is also thought to have a role in nasopharyngeal carcinoma development as well as glutamate production. BCAT1 is known to have interactions with SMAD5, HSPD1, UBC, PIN1 and AADAT. Disease and disorder research has been conducted in relation to BCAT1 and hyperleucine-isoleucinemia, hypervalinemia or hyperleucine-isoleucinemia, and hyperleucinemia-isoleucinemia or hypervalinemia.

Long Name

Branched Chain Amino Acid Transaminase 1

Alternate Names

BCAT-1, BCATC, BCT1, EC 2.6.1.42, ECA39, PNAS121, PP18

Gene Symbol

BCAT1

Additional BCAT1 Products

Product Documents for BCAT1 Antibody (OTI3F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BCAT1 Antibody (OTI3F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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