BCKDHA Antibody (BLR060F)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14681
Recombinant Monoclonal
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR060F
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
surrounding serine 292
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for BCKDHA Antibody (BLR060F)
Immunoprecipitation: BCKDHA Antibody (BLR060F) [NBP3-14681]
Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.Simple Western: BCKDHA Antibody [NBP3-14681] -
Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.Simple Western: BCKDHA Antibody [NBP3-14681] -
Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.Applications for BCKDHA Antibody (BLR060F)
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: BCKDHA
Alternate Names
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDE1A, BCKDH E1-alpha, branched chain keto acid dehydrogenase E1, alpha polypeptide, branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease), Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide), FLJ45695, MSU, MSUD1, OVD1A
Gene Symbol
BCKDHA
Additional BCKDHA Products
Product Documents for BCKDHA Antibody (BLR060F)
Product Specific Notices for BCKDHA Antibody (BLR060F)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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