Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11C9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BCKDK Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCKDK (NP_005872) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCKDK Antibody (OTI11C9) - Azide and BSA Free

Western Blot: BCKDK Antibody (OTI11C9)Azide and BSA Free [NBP2-71573]

Western Blot: BCKDK Antibody (OTI11C9)Azide and BSA Free [NBP2-71573]

Western Blot: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCKDK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCKDK.
Immunocytochemistry/ Immunofluorescence: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573]

Immunocytochemistry/ Immunofluorescence: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573]

Immunocytochemistry/Immunofluorescence: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCKDK.
Immunohistochemistry: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573]

Immunohistochemistry: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573]

Immunohistochemistry: BCKDK Antibody (OTI11C9) - Azide and BSA Free [NBP2-71573] - Staining of paraffin-embedded Human endometrium tissue using anti-BCKDK mouse monoclonal antibody.
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Applications for BCKDK Antibody (OTI11C9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCKDK

BCKDK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage

Alternate Names

BCKDHKIN, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase, EC 2.7.11, EC 2.7.11.4, mitochondrial

Gene Symbol

BCKDK

Additional BCKDK Products

Product Documents for BCKDK Antibody (OTI11C9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCKDK Antibody (OTI11C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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