BCKDK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86052

Novus Biologicals, part of Bio-Techne
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NBP1-86052
NBP1-86052-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BCKDK Antibodies »

Product Summary for BCKDK Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCKDK Antibody

Western Blot: BCKDK Antibody [NBP1-86052]

Western Blot: BCKDK Antibody [NBP1-86052]

Western Blot: BCKDK Antibody [NBP1-86052] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052]

Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052]

Immunohistochemistry-Paraffin: BCKDK Antibody [NBP1-86052] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Western Blot: BCKDK Antibody [NBP1-86052]

Western Blot: BCKDK Antibody [NBP1-86052]

Western Blot: BCKDK Antibody [NBP1-86052] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
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Applications for BCKDK Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCKDK

BCKDK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage

Alternate Names

BCKDHKIN, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase, EC 2.7.11, EC 2.7.11.4, mitochondrial

Gene Symbol

BCKDK

Additional BCKDK Products

Product Documents for BCKDK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BCKDK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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