Bcl-2 Antibody (124) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34513

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 124

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Bcl-2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein (Uniprot: P10415 )

Reactivity Notes

Does not react with Mouse. Others-not known

Localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Specificity

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Marker

Apoptosis & Follicular Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34443).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Bcl-2 Antibody (124) - Azide and BSA Free

Western Blot: Bcl-2 Antibody (124)Azide and BSA Free [NBP2-34513]

Western Blot: Bcl-2 Antibody (124)Azide and BSA Free [NBP2-34513]

Western Blot: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513] - Western Blot Analysis of MCF-7 cells using Bcl-2 Antibody (124).
Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (124).
Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) - Azide and BSA Free [NBP2-34513] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (124). Note nuclear membrane & cytoplasmic staining.

Applications for Bcl-2 Antibody (124) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (124) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody (124) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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