Bcl-2 Antibody (8C8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33313
![Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]](https://resources.bio-techne.com/images/products/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0003.jpg "Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Porcine, Monkey, Mouse (Negative), Rat (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8C8
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein. (Uniprot: P10415 )
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Specificity
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Marker
Apoptosis & Follicular Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29630).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Bcl-2 Antibody (8C8) - Azide and BSA Free
Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]
Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Western blot of Bcl-2 in human A) HeLa B) A549 and C) MCF7 Lysate using Bcl-2 Monoclonal Antibody (8C8)![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313]](https://resources.bio-techne.com/images/products/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33313-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313]")
Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313]
Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Formalin-fixed paraffin-embedded human tonsil stained with Bcl-2 Monoclonal Antibody (8C8) Note cell membrane and cytoplasmic staining![Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]](https://resources.bio-techne.com/images/products/Bcl-2-Antibody-8C8-Azide-and-BSA-Free-Western-Blot-NBP2-33313-img0001.jpg "Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]")
Western Blot: Bcl-2 Antibody (8C8)Azide and BSA Free [NBP2-33313]
Western Blot: Bcl-2 Antibody (8C8) - Azide and BSA Free [NBP2-33313] - Analysis of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.Applications for Bcl-2 Antibody (8C8) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bcl-2
Long Name
B Cell Lymphoma/Leukemia 2
Alternate Names
Bcl2
Gene Symbol
BCL2
Additional Bcl-2 Products
Product Documents for Bcl-2 Antibody (8C8) - Azide and BSA Free
Product Specific Notices for Bcl-2 Antibody (8C8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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