Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Bcl-2 related protein A1 Antibodies »

Product Summary for Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human BCL2A1 (NP_004040) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free

Western Blot: Bcl-2 related protein A1 Antibody (OTI3D10)Azide and BSA Free [NBP2-70246]

Western Blot: Bcl-2 related protein A1 Antibody (OTI3D10)Azide and BSA Free [NBP2-70246]

Western Blot: Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free [NBP2-70246] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bcl-2 related protein A1.

Applications for Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-2 related protein A1

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Alternate Names

BCL2A1, Bcl2L5, Bfl-1, GRS

Gene Symbol

BCL2A1

Additional Bcl-2 related protein A1 Products

Product Documents for Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bcl-2 related protein A1 Antibody (OTI3D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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