Bcl-2 related protein A1 Antibody (SC05-42)

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563]

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Mouse kidney tissue with Rabbit anti-BCL2A1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563]

Flow Cytometry: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of 293 cells with Bfl1(BCL2A1) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563]

Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded human kidney tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.