Bcl-6 Antibody (BCL6/1475)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59596
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Protein Array, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BCL6/1475
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human bcl-6 protein fragment (around aa256-389) (exact sequence is proprietary) (Uniprot: P41182)
Localization
Nuclear
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Marker
Follicular Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Positive Control: Raji or Ramos cells. Tonsil
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59597)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59597)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bcl-6 Antibody (BCL6/1475)
Protein Array: Bcl-6 Antibody (BCL6/1475) [NBP2-59596] - Bcl-6 Antibody [NBP2-59596] - Using more than 19000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary AB) produces when binding to a protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of ab to its intended target. An Ab is considered specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29.
![Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]](https://resources.bio-techne.com/images/products/Bcl-6-Antibody-BCL6-1475-Western-Blot-NBP2-59596-img0010.jpg "Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]")
Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]
Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596] - Western Blot Analysis (A) Recombinant Protein (B) HepG2 cell lysate Bcl-6 Antibody (BCL6/1475).![Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]](https://resources.bio-techne.com/images/products/Bcl-6-Antibody-BCL6-1475-Western-Blot-NBP2-59596-img0002.jpg "Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]")
Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596]
Western Blot: Bcl-6 Antibody (BCL6/1475) [NBP2-59596] - Analysis (A) Recombinant Protein (B) HepG2 Cell lysateUsing bcl-6 Monoclonal Antibody (BCL6/1475).Applications for Bcl-6 Antibody (BCL6/1475)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Protein Array
1:100-1:2000
Western Blot
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bcl-6
Long Name
B Cell Lymphoma/Leukemia 6
Alternate Names
Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51
Gene Symbol
BCL6
UniProt
Additional Bcl-6 Products
Product Documents for Bcl-6 Antibody (BCL6/1475)
Product Specific Notices for Bcl-6 Antibody (BCL6/1475)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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