BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13763

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-BCL2L2-1A4

Concentration

0.2 mg/ml

View all Bcl-w Antibodies »

Product Summary for BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

Immunogen

Recombinant human full-length BCL-W/BCL2L2 protein (Uniprot: Q92843)

Localization

Cytoplasm, Mitochondrion membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14166)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 22-30kDa

Scientific Data Images for BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Immunocytochemistry/Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Flow Cytometry: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Flow Cytometry: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763] - Flow cytometric analysis of PFA-fixed Raji cells. BCL-W/BCL2L2 antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763]

Immunocytochemistry/Immunofluorescence: BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) [NBP3-13763] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained using BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
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Applications for BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-w

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.

Long Name

B Cell Lymphoma/Leukemia w

Alternate Names

BCL2L2, Bclw

Gene Symbol

BCL2L2

UniProt

Q92843

Additional Bcl-w Products

Product Documents for BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BCL-W/BCL2L2 Antibody (PCRP-BCL2L2-1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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