bcl-x Antibody (2H12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11881

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2H12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bcl-x Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence (Uniprot: Q07817)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for bcl-x Antibody (2H12)

Western Blot: bcl-x Antibody (2H12) [NBP3-11881]

Western Blot: bcl-x Antibody (2H12) [NBP3-11881]

Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.

Applications for bcl-x Antibody (2H12)

Application
Recommended Usage

Western Blot

1 - 5 ug/ml
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

UniProt

Q00534 (Human)

Additional Bcl-x Products

Product Documents for bcl-x Antibody (2H12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for bcl-x Antibody (2H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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