bcl-x Antibody (5E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26480
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5E9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for bcl-x Antibody (5E9)
Immunogen
A synthesized peptide derived from Human Bcl-x [UniProt Q07817]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for bcl-x Antibody (5E9)
Immunohistochemistry: bcl-x Antibody (5E9) [NBP3-26480] -
Immunohistochemistry: bcl-x Antibody (5E9) [NBP3-26480] - Image of bcl-x Antibody (5E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: bcl-x Antibody (5E9) [NBP3-26480] -
Western Blot: bcl-x Antibody (5E9) [NBP3-26480] - Positive Western Blot detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate.All lanes: bcl-x Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 27, 19, 26 kDa
Observed band size: 30 kDa
Applications for bcl-x Antibody (5E9)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Bcl-x
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
Additional Bcl-x Products
Product Documents for bcl-x Antibody (5E9)
Product Specific Notices for bcl-x Antibody (5E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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